mail
editor@ijphdt.com
whatsapp
+919343055451
e-ISSN: 3049-1630

International Journal of Pharmacognosy and Herbal Drug Technology

Published

Standardization and Quality Control of Polyherbal Formulations Using HPLC and UV-Vis Spectroscopy

Published in IJPHDT, Vol-2, Issue-8, Aug-2025 (Vol. 2, Issue 8, 2025)

Standardization and Quality Control of Polyherbal Formulations Using HPLC and UV-Vis Spectroscopy - Issue cover

Abstract

Complex phytochemical composition of polyherbal formulations which are dominant in traditional medicine systems makes it hard to standardize them. The research discusses a confirmed analytical method of High-Performance Thin-Layer Chromatography (HPTLC) and Ultraviolet-Visible (UV-Vis) Spectroscopy to standardize and guarantee the quality control of polyherbal formulation. Validated HPTLC methods based on ICH guidelines were used to quantify selected marker compounds-gallic acid, quercetin, mangiferin and curcumin- in terms of linearity, accuracy, precision, specificity, limits of detection (LOD) and limits of quantification (LOQ). In tandem with this, UV-Vis absorption spectra and calibration curves were developed to quantitatively analyse substances using Beer Lambert law with the instrumental limitations in mind (e.g. stray light and bandwidth of the dual wavelength approach). These results obtained high correlation coefficients (>0.999), detection limit in nanogram range and accurate quantification of significant bioactive markers. The combined workflow provides a sound, reproduceable and cost-efficient platform of quality control of complex herbal blends. The study helps to advance the use of polyherbal preparations because it helps to mediate between traditional medicine approaches and contemporary analytical standards.

Authors (2)

Jaidev Kumar

Department of Chemistry, Hariom Saraswati P.G. College, Dhanauri, Haridwar, Uttarakhand, Pin-247667

View all publications →

Tusar Bajpai

Department of Chemistry, Hariom Saraswati P.G. College, Dhanauri, Haridwar, Uttarakhand, Pin-247667

View all publications →

Download Article

PDF

Best for printing and citation

File size: 0.0 MB
Format: PDF

Article Information

Article ID:
IJPHD-00000134
Paper ID:
IJPHD-01-000134

How to Cite

, J. & , T. (2025). Standardization and Quality Control of Polyherbal Formulations Using HPLC and UV-Vis Spectroscopy. International Journal of Pharmacognosy and Herbal Drug Technology, 2(8), xx-xx. https://ijphdt.scholarjms.com/articles/65

Article Actions

Article Impact

Views:1,247
Downloads:1,099
Citations:106
Altmetric Score:40
1,247
Article Views
1,099
Downloads
106
Citations
12
Social Shares

More from this Issue

Formulation and Evaluation of Herbal Sunscreen Lotion Enriched with Natural Plant Extracts

Background: Prolonged exposure to ultraviolet (UV) radiation may result in sunburn, accelerated aging, cutaneous damage, and potentially, carcinoma of the skin. While effective, synthetic sunscreens are frequently linked to dermal irritation and possible toxicity. Herbal sunscreens, formulated with natural, plant-derived compounds, present a more secure and ecologically sound alternative. Objective: To develop and evaluate a herbal sunscreen lotion utilizing plant extracts possessing established UV-protective, antioxidant, and skin-soothing properties. Materials and Methods: Herbal extracts derived from Aloe vera, Watermelon, Sandalwood, and Turmeric were selected based on their photoprotective and antioxidant capabilities. The lotion base was prepared utilizing conventional emulsification methodologies. Physicochemical properties, including pH, viscosity, spreadability, and stability, in-vitro sun protection factor determined via UV spectrophotometry were evaluated for the formulated herbal sunscreen lotion. Results: The herbal sunscreen lotion exhibited acceptable spreadability, stability under diverse storage conditions, and an appropriate pH (5.5–6.5). Confirmation of effective UV-B protection was established by the moderate range (SPF 15–25) of the in-vitro SPF value. The presence of significant antioxidant activity suggested the potential for skin protection from oxidative damage. Conclusion: The herbal sunscreen cream exhibits potential antioxidant properties in conjunction with ultraviolet (UV) protection. It serves as a safe, natural, and efficient alternative to chemical sunscreens, demonstrating commercialization potential.

Anjali Sahu, Suman ShrivastavaRead more →

Phytochemical Screening and Anti-Ageing Potential of Herbal Extracts in A Topical Cream Formulation

Background: Calendula, chamomile, and butterfly pea contain a high concentration of antioxidants that rejuvenate skin and provide relief from irritation. Based on this information, we can assume that extracts of these plants can be used to make a sustainable anti-aging cream as a natural alternative to existing skincare products. Objective: This research aims to formulate a topical cream using extracts of calendula, chamomile, and butterfly pea. The work also includes tests of the phytochemical profile and evaluation parameters of the cream. Materials and Methods: In this study, we assessed the content of flavonoids, phenolics, tannins, saponins, and alkaloids by performing qualitative phytochemical screening on methanolic extracts of Clitoria ternatea, Calendula officinalis, and Matricaria chamomilla. Using standard methods, we emulsified the mixed herbal extracts to produce an oil-in-water cream. Once prepared, the cream formulation was evaluated for organoleptic properties, pH, viscosity, homogeneity, spreadability, and stability under a variety of storage conditions. Results: All three herbal extracts were shown to have significant quantities of phenolic compounds and flavonoids upon phytochemical analysis. Additionally, the formulated cream proved to have desirable organoleptic qualities that include a pH of 4.5- 5.2, stable viscosity, and good spreadability. It also proved to remain homogeneous with no evidence of phase separation or microbial growth over the duration of the 30-day stability study. Conclusion: Anti-aging cream formulation using butterfly pea, calendula, and chamomile extracts exhibited promising physical, chemical, and stability profiles. The phytochemical components support the viability of its use as a natural anti-aging formulation. Further studies to assess its biological efficacy are recommended.

Suman Shrivastava, Laxmi Sen, Akash DewanganRead more →

Polyherbal Sunscreen Cream: Formulation, Physicochemical Evaluation, And In Vitro SPF Determination

Background: Herbal bioactives with antioxidant and UV-protective properties are increasingly favored in sunscreen formulations for their safety and efficacy. This study focuses on the development and optimization of a polyherbal sunscreen cream incorporating Camellia sinensis (green tea), Citrus sinensis (orange peel), Punica granatum (pomegranate peel), Curcuma longa (turmeric) extracts in an oil-in-water emulsion base. Objective: To formulate and evaluate a stable, non-greasy polyherbal sunscreen cream and identify the optimal formulation in terms of SPF, texture, spreadability, and stability. Methods: Three formulations (F1–F3) were prepared using a fixed base of stearyl alcohol, cetyl alcohol, emulsifying wax, olive oil, glycerin, and parabens, with varying concentrations of herbal extract blend (6% to 10% w/w). The creams were evaluated for physical characteristics, pH, viscosity, spreadability, in-vitro SPF, and stability under accelerated conditions (centrifugation, thermal cycling, and long-term storage). Results: All formulations were smooth, yellow-green, optically homogeneous, and free from grittiness. pH values remained skin-compatible (6.05–6.15), and no phase separation was observed during stability testing. F3, containing the highest concentration of polyherbal extract and oil phase, exhibited the highest SPF value of 22.7 ± 0.6, a viscosity of 17,600 ± 210 cP, and a spreadability of 5.2 ± 0.2 cm, indicating a rich texture with high photoprotective capacity. All formulations passed centrifugation and thermal cycling tests and maintained long-term stability over 90 days. Conclusion: Among the three tested formulations, F3 was found to be the most effective due to its superior SPF value, acceptable spreadability, and excellent stability. This study highlights the potential of polyherbal extracts as safe and effective ingredients in natural sunscreen formulations, suitable for further in vivo testing and commercial development.

Morlin Toppo, Saurabh ShrivastavaRead more →