MTT assay

One of the leading causes of cancer-related death for women worldwide is breast cancer. Natural substances obtained from therapeutic plants, particularly terpenoids, have garnered considerable interest as potential sources for pharmaceuticals. This study aims to determine which plant-based terpenoids, when tested in vitro against MCF-7 and MDA-MB-231, exhibit anti-cancer properties. Terpenoids were extracted using conventional chromatographic methods, and their identification was confirmed using spectroscopic analysis. We used fluorescence microscopy and flow cytometry to detect apoptotic effects and the MTT assay to measure cytotoxicity. The findings demonstrated that the dose affected the effects of apoptosis induction and cancer cell growth inhibition. The authors of the study believe that terpenoids from plants have a lot of potential as a treatment for breast cancer.