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International Journal of Pharmacognosy and Herbal Drug Technology

Published

Authentication and Adulteration Detection of Herbal Raw Materials Using DNA Barcoding and FTIR Fingerprinting

Published in IJPHDT, Vol-2, Issue-11, Nov-2025 (Vol. 2, Issue 11, 2025)

Authentication and Adulteration Detection of Herbal Raw Materials Using DNA Barcoding and FTIR Fingerprinting - Issue cover

Abstract

The growing demand of herbal medicine in the world market has heightened the chances of adulteration and misidentification thus undermining safety, efficacy and quality. This study set out to authenticate three herbal raw materials waste components, namely, Gymnema sylvestre, Curcuma longa, and Trigonella foenum-graecum, and identify adulteration by DNA barcoding and Fourier Transform Infrared (FTIR) spectroscopy against pharmacological analysis in Wistar rats to determine biological efficacy. DNA barcoding was precise in determining is genuine species and differentiating them with adulterated samples whereas FTIR fingerprinting identified the presence of chemical adulterants and correlated with changes in therapeutic capability. Pharmacological tests identified that genuine extracts had a significant effect in lowering the level of blood glucose and had better effects than commercial and adulterated extracts, the level of which declined proportionally to the extent of adulteration. The results prove that a combination of molecular, chemical, and pharmacological techniques is a stable and successful strategy of quality control, authentication, and safety testing of herbal raw materials, and purity may play a key role in the therapeutic efficacy.

Authors (5)

B Sabana Sabana

Department of Biochemistry and Bioinformatics Dr MGR Janaki college for women, Adyar, Chennai 28

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T Uma Venkatalakshmi Venkatalakshmi

Department of Biochemistry and Bioinformatics, Dr MGR Janaki college for women, Adyar, Chennai 28

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Anjum. A Anjum

Justice Basheer Ahmed Sayeed College for Women (Autonomous), Chennai

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R.Ramya Ramya

Department of Biochemistry, J.B.A.S. College for Women Teynampet, Chennai -18

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Bhuvaneswari.G G

Department of Biochemistry, Annai Veilankanni’s College for Women

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Article Information

Article ID:
IJPHD-00000201
Paper ID:
IJPHD-01-000201

How to Cite

Sabana, B. & T Uma Venkatalakshmi Venkatalakshmi & A, A. & Ramya & G (2025). Authentication and Adulteration Detection of Herbal Raw Materials Using DNA Barcoding and FTIR Fingerprinting. International Journal of Pharmacognosy and Herbal Drug Technology, 2(11), xx-xx. https://ijphdt.scholarjms.com/articles/86

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