Vaishali Sharma

Rauwolfia serpentina is a well-known medicinal plant in traditional medicine. It is noted for containing a high concentration of alkaloids and being able to help alleviate symptoms associated with high blood pressure and neurological diseases. The goal of this study was to employ a systematic phytochemical method to separate and characterise the structure of the bioactive alkaloids present in the roots of R. serpentina. We used methanol to extract the dried root powder and then split it into smaller parts using hexane, chloroform, ethyl acetate, butanol, and water. The chloroform fraction contained the highest concentration of alkaloids (2.68%) and was subsequently separated into five distinct alkaloids using column chromatography. TLC profiling showed that these chemicals were Reserpine, Ajmaline, Yohimbine, Serpentine, and Ajmalicine, and FTIR, NMR (^1H and ^13C), and Mass Spectrometry verified this. The statistical analysis (ANOVA) revealed significant differences in alkaloid content between the solvent systems (p = 0.001), with chloroform being the most effective extractant. The results confirm that R. serpentina is rich in phytochemicals and provide a proven method for isolating natural products. This has implications for the discovery and development of further medications